TY - JOUR AU - Bouraima, BAMBOLA AU - Alban, HOUNGBEME Gouton AU - Louis, FAGBOHOUN AU - Sèdami, MEDEGAN AU - Fernand, GBAGUIDI Ahokanou PY - 2020/01/09 Y2 - 2024/03/28 TI - PURIFICATION AND BIOLOGICAL ACTIVITIES OF COMBINATION OF HEMI-SYNTHESIZED THIOSEMICARBAZONES OF MITRACARPUS SCABER ZUCC JF - Universal Journal of Pharmaceutical Research JA - Univ J Pharm Res VL - 4 IS - 6 SE - Research Articles DO - 10.22270/ujpr.v4i6.335 UR - https://www.ujpr.org/index.php/journal/article/view/335 SP - AB - <p><strong>Objective: </strong>The harvested <em>Mitracarpus Scaber</em> plants are identified in the national herbarium and registered under the number AA. 6252/HLB. During this work, three crude extracts are prepared from three organic solvents, namely dichloromethane; ethanol and hydroethanol in 50/50 v/v proportion.</p><p><strong>Methods: </strong>The respective alkaloid extracts obtained from the corresponding crude extracts served as substrates for the hemi synthesis of thiosemicarbazone totals from thiosemicarbazides. The three hemi-synthesis products obtained were tested on eight (08) strains of germs, namely <em>E. coli</em> ATCC 25922, <em>S. aureus</em> ATCC 25923, <em>E. faecalis</em> ATCC 22921, <em>P. aeruginosa</em> ATCC 27853<em>, C. albicans </em>ATCC 10231, <em>S. typhi</em>, <em>K. pneumoniae</em> and <em>Dermatophilus</em> 146.</p><p><strong>Results: </strong>The ethanolic extract of thiosemicarbazones exhibited the best bioactive activity and was found to be the most selective. By a series of bioguided chromatographies: TLC thin layer chromatography; CPA atmospheric pressure chromatography and medium pressure liquid chromatography. MPLC (medium pressure liquid chromatography), separation on dextran gel: Sephadex® LH20). The use of available spectral data, cross-checked with that of the literature, made it possible to identify and purify three (03) molecules of thiosemicarbazones, making it possible to study the biological activity of their combination.</p><p><strong>Conclusion:</strong> On the basis of results, this work has made it possible to confirm the concept which affirms that medicinal plants are libraries of several thousand molecules.</p><p>                            <img src="https://ujpr.org/public/site/images/drkk/r72.gif" /></p><p><strong>Peer Review History: </strong></p><p><strong>Received </strong>2 November 2019;<strong>   Revised </strong>20 December;<strong> Accepted </strong>5 January,<strong> Available online </strong>15 January 2020</p><p style="text-align: justify;"><strong>Academic Editor: </strong><strong>Ahmad Najib<sub><a href="https://orcid.org/0000-0001-5055-7964" target="_blank" rel="noopener"><img src="https://ujpr.org/public/site/images/editor/orcid22.jpg" /></a></sub></strong>, Universitas Muslim Indonesia,  Indonesia, <em>ahmad.najib@umi.ac.id</em></p><p><strong>Received file:<a href="http://ujpr.org/2019/4-6-r7.pdf" target="_blank" rel="noopener"><img src="https://ujpr.org/public/site/images/drkk/blue_23983.gif" /></a>                Reviewer's Comments:<a href="http://ujpr.org/2019/4-6-r7-comments.doc" target="_blank" rel="noopener"><img src="https://ujpr.org/public/site/images/drkk/download_logo_r_29189.gif" /></a></strong></p><p><strong>Average Peer review marks at initial stage:</strong> 5.5/10</p><p><strong>Average Peer review marks at publication stage:</strong> 8.0/10</p><p><strong>Reviewer(s) detail:</strong></p><p><strong>Prof. Dr. Ali Gamal Ahmed Al-kaf<sub><a href="https://orcid.org/0000-0002-4673-7281" target="_blank" rel="noopener"><img src="https://ujpr.org/public/site/images/editor/orcid22.jpg" /></a></sub></strong>, Sana'a university, Yemen, <em>alialkaf21@gmail.com</em></p><p><strong>Prof. Dr. Amani S. Awaad<sub><a href="https://orcid.org/0000-0001-7910-6841" target="_blank" rel="noopener"><img src="https://ujpr.org/public/site/images/editor/orcid22.jpg" /></a></sub></strong>, Prince Sattam Bin Abdulaziz University, Al-Kharj. KSA., <em>amaniawaad@hotmail.com</em></p><p><strong>Similar Articles:</strong></p><p><a href="http://www.ujpr.org/index.php/journal/article/view/12" target="_blank" rel="noopener">ANTIFUNGAL, CYTOTOXIC AND PHYTOTOXICITY OF AERIAL PART OF RANUNCULUS MURICATUS </a></p><p><a href="http://www.ujpr.org/index.php/journal/article/view/26" target="_blank" rel="noopener">ANTI-INFLAMMATORY AND ANTI-OXIDANT ACTIVITIES OF METHANOL EXTRACT OF BAPHIA NITIDA </a></p> ER -