IN VITRO GENOTOXICITY OF THREE PLANTS FROM ASTERACEAE FAMILY IN HUMAN LYMPHOCYTES CULTURES

  • M. Kinan Aljaja Dept. Of Pharmacognosy and medicinal plants, Faculty of Pharmacy, University of Aleppo, Syria.
  • Adawia Kitaz Dept. Of Pharmacognosy and medicinal plants, Faculty of Pharmacy, University of Aleppo, Syria.
  • Raghda Lahdo Dept. Of Biochemistry and Microbiology, Faculty of Pharmacy, University of Aleppo, Syria.

Abstract

Background: Onopordum carduiforme, Centaurea verutum, and Achillea santolina are medicinal plants grown in Syria and commonly used in traditional medicine. Such as antibacterial, antioxidant and anticancer properties. However, the genotoxic effects of these plants have not been studied.


Aim and objective: the aim of this study was to evaluate the genotoxic effects of hydroethanolic extracts of these plants on human lymphocyte cultures model by evaluating the cell proliferation, determination of mitotic index (MI), and their effects on chromosomes.


Methods: the hydroethanolic extracts of the aerial parts of the three plants were extracted using an Ultrasonic bath. Then the genotoxic effects of hydroethanolic extracts of these plants on human lymphocyte cultures was conducting by determination of mitotic index (MI).


Results: the results showed that all three plants decreased non-significantly the mean of mitotic index in comparison with negative control (normal MI) (p>0.05) at concentrations (1, 3, 5 mg/ml) and the mitotic index values ranged was between (2.25±0.07 and 3.3±0.28). However, C. verutum showed the lowest mitotic index (3±0.14 at 1 mg/ml) and (2.25±0.07 at 5 mg/ml), and did not induce chromatid or chromosome breaks or gaps.


Conclusion: these preliminary results on cytotoxicity and mutagenicity of these plants provide valuable information about the safety of using them in alternative medicine.


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Peer Review History:


Received: 11 November 2021; Revised: 13 December; Accepted: 28 December, Available online: 15 January 2022


Academic Editor:  Dr. A.A. Mgbahurikeorcid22.jpg, University of Port Harcourt, Nigeria, amaka_mgbahurike@yahoo.com


UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. 


Received file:blue_23983.gif                Reviewer's Comments:download_logo_r_29189.gif


Average Peer review marks at initial stage: 6.0/10


Average Peer review marks at publication stage: 7.0/10


Reviewers:


orcid22.jpgAhmad Najib, Universitas Muslim Indonesia, Makassar, Indonesia, ahmad.najib@umi.ac.id


orcid22.jpgDr. Dennis Amaechi, MrsFoluBabade Mini Estate , Flat 5 by Old Soldiers Quarter, Sabongari/Bwari, Abuja- Federal Capital Territory, Nigeria. amaechitoexcel@yahoo.com 


orcid22.jpgDr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, sangeetha@utar.edu.my


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Keywords: Achillea, Centaurea, human lymphocytes cultures, karyotype, mitotic index (MI), Onopordum

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How to Cite
Aljaja, M. K., A. Kitaz, and R. Lahdo. “IN VITRO GENOTOXICITY OF THREE PLANTS FROM ASTERACEAE FAMILY IN HUMAN LYMPHOCYTES CULTURES”. Universal Journal of Pharmaceutical Research, Vol. 6, no. 6, Jan. 2022, doi:https://doi.org/10.22270/ujpr.v6i6.692.
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Research Articles