CYTOTOXIC ACTIVITIES IN VITRO OF FLOWER EXTRACTS OF THREE SPECIES OF ALOE GROWING IN YEMEN:ALOE RUBROVIOLACEAE, ALOE VERA AND ALOE SABAEA, AGAINST ELEVEN TYPES OF CANCER CELL LINES

  • Hassan Mohammed Al-Mahbashi Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine and Health Sciences, Sana’a University, Yemen.
  • Amina M El Shaibany Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine and Health Sciences, Sana’a University.
  • Mohammad Abobakr Al-Ghazali Department of Pharmacology & Biology Sciences, College of Pharmacy, National University of Science and Technology, Muscat, Oman. Department of Pharmacology &Toxicology, College of Medicine and Health Sciences Thamar University, Yemen.
  • Hassan A. Al-Shamahy Medical Microbiology and Clinical Immunology Department, Faculty of Medicine and Health Sciences, Sana’a University, Republic of Yemen.
  • Azhar Azher Mohammed Al-Ankoshy Departement of physiology, Jabir Ibn Hayyan Medical University, Faculty of Medicine,Iraq.

Abstract

 Background and aims: Natural products, in particular plant extracts, have opened up great chance in the area of drug progress owing to their chemical variety. The Aloe genus has long been known to be used for medicinal uses in countless parts of the world. This study was planned to inspect the phytochemicals and anti-cancer capabilities of Aloe rubroviolaceae, Aloe vera and Aloe sabaea flowers.


Materials and Methods: Three types of ethanolic extracts of plants traditionally used in Yemen to treat a variety of diseases have been tested in vitro for their potential anticancer activity on different human cancer cell lines. The cytotoxic activity of the ethanolic extracts of tested plants was determined using eleven strains of human cancer cells, namely: MCF-7 (breast cancer), PC-3 (prostate cancer), HEP-2(human epithelial carcinoma), MNFS-60 (myelogenous leukemia), CACO (intestinal cancer), A-549 (lung adenocarcinoma), HeLa (cervical cancer),RD (rhabdomyosarcoma), HepG2 (hepatocellular carcinoma), HCT-116 (colon cancer),  and CHO-K1(Chinese hamster ovary). A colorimetric sulforhodamine B assay was applied to assess the in vitro cytotoxic activity of various extracts. Growth inhibition of 50% (IC50) for each extract was calculated from the optical density of treated and untreated cells. Doxorubicin, a broad-spectrum anticancer drug was used as a positive control.


Results: More interesting cytotoxic activity was observed for Aloe vera extract more than Aloe sabaea and Aloe rubroviolaceae, extract


Conclusions: This study presents an initial screening for anti-proliferative activity of a variety of Aloe species flowers extracts on diverse cancer cell lines. Different extracts of Aloe species significantly inhibit the growth of various cancer cell lines  in a concentration-dependent manner. Advance researches are necessary to understand the possible mechanism(s) of action of these extract on a variety of cancer cells and separation of active phyto-chemicals.


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Peer Review History:


Received: 18 July 2021; Revised: 17 August; Accepted: 8 September, Available online: 15 September 2021


Academic Editor:  Dr. Muhammad Zahid Iqbalorcid22.jpg, AIMST University, Malaysia, drmmziqbal@gmail.com


UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. 


Received file:blue_23983.gif                Reviewer's Comments:download_logo_r_29189.gif


Average Peer review marks at initial stage: 6.5/10


Average Peer review marks at publication stage: 7.5/10


Reviewers:


Dr. U. S. Mahadeva Raoorcid22.jpg, Universiti Sultan Zainal Abidin, Terengganu Malaysiaraousm@gmail.com


Dr. Nazim Hussainorcid22.jpg, BFIT, Dehradun, Uttarakhand, India, nhussain116@gmail.com


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Keywords: Aloe species, cytotoxic activities, flower extracts, in vitro, Yemen

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How to Cite
Al-Mahbashi, H. M., A. M. E. Shaibany, M. A. Al-Ghazali, H. A. Al-Shamahy, and A. A. M. Al-Ankoshy. “CYTOTOXIC ACTIVITIES IN VITRO OF FLOWER EXTRACTS OF THREE SPECIES OF ALOE GROWING IN YEMEN:ALOE RUBROVIOLACEAE, ALOE VERA AND ALOE SABAEA, AGAINST ELEVEN TYPES OF CANCER CELL LINES”. Universal Journal of Pharmaceutical Research, Vol. 6, no. 4, Sept. 2021, doi:https://doi.org/10.22270/ujpr.v6i4.645.
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