PORPHYROMONAS GINGIVALIS: BIOFILM FORMATION, ANTIMICROBIAL SUSCEPTIBILITY OF ISOLATES FROM CASES OF LOCALIZED AGGRESSIVE PERIODONTITIS (LAP)

  • Husian Shogaa Al-Deen Orthodontics, Pedodontics and Prevention Department Faculty of Dentistry, Sana'a University, Yemen.
  • Azhar Azher Mohammed Al-Ankoshy Physiology Department, Jabir ibnHayyan Medical University, Faculty of Medicine, Iraq.
  • Mohammed Mohammed Ali Al-Najhi Orthodontics, Pedodontics and Prevention Department Faculty of Dentistry, Genius University for Sciences and Technology, Dhamar city, Republic of Yemen.
  • Tagrid Ahmed Al-Kabsia Department of Maxillo-Facial, Faculty of Dentistry, Sana’a University, Republic of Yemen.
  • Khaled A AL-Haddad Orthodontics, Pedodontics and Prevention Department Faculty of Dentistry, Sana'a University, Yemen.
  • Ameen Abdullah Yahya Al-Akwa Orthodontics, Pedodontics and Prevention Department Faculty of Dentistry, Sana'a University, Yemen.
  • Hassan Abdulwahab Al-Shamahy Department of Basic Sciences, Faculty of Dentistry, Sana’a University, Republic of Yemen.
  • Mohammed A Al-labani Orthodontics, Pedodontics and Prevention Department Faculty of Dentistry, Sana'a University, Yemen.

Abstract

Background: No information is available on the antimicrobial sensitivity pattern and guidelines for oral antibiotic therapy for Yemeni patients for those with localized aggressive periodontitis (LAP) due to Porphyromonas gingivalis, a condition that often requires complementary antibiotic treatment.


Aim: The primary aim of the current study was to examine the antibiotic patterns and the potential relationship between P. gingivalis biofilm formation and the incidence of antibiotic resistance of clinical isolates on a group of antibiotics commonly used in oral/systemic therapy.


Subjects and Methods: The study included 30 clinically diagnosed patients, and 30 strains of P. gingivalis were isolated from them.  Microbial sampling, isolation, and identification of bacteria were performed using culture methods appropriate to anaerobic species. Biofilm production was evaluated by the phenotypic method, that is, tissue culture methods (TCPM). Also; each isolate was tested against 12 antibiotics using the disc diffusion method.


Results: After isolated P. gingivalis were subjected to biofilm detection by TCP method, 7 (23.3%) showed high, 6 (20%) moderate, while 17 (56.7%) showed non/weak biofilm-forming ability. P. gingivalis  biofilms showed a higher resistance rate than forming non/weak biofilms e.g amoxicillin (92.3% vs 64.7%, p = 0.08), azithromycin (58.8% vs 11.7, p =0.003), metronidazole (76.9% vs 29.4%, p = 0.08), 0.01) and clindamycin (84.6% vs 47.1, p = 0.03).


Conclusion: It was found that the drug-resistant factor in P. gingivalis isolates is associated with the formation of P. gingivalis biofilm. Even though the current results show a high sensitivity result for P. gingivalis strains, some resistance has been observed. Antibiotic resistance patterns can modify over the years, make susceptibility testing essential and to promote careful choice of preliminary antibiotic treatment, the same as an adjuvant to endodontic therapy.


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Peer Review History:


Received: 9 July 2021; Revised: 12 August; Accepted: 6 September, Available online: 15 September 2021


Academic Editor:  Dr. Ali Abdullah Al-yahawiorcid22.jpg, Al-Razi university, Department of Pharmacy, Yemen, alyahawipharm@yahoo.com


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Received file:blue_23983.gif                Reviewer's Comments:download_logo_r_29189.gif


Average Peer review marks at initial stage: 6.0/10


Average Peer review marks at publication stage: 7.0/10


Reviewers:


Dr. Rawaa Souhil Al-Kayaliorcid22.jpg, Aleppo University, Syriarawah67@hotmail.com


Dr. Wadhah Hassan Ali Edreesorcid22.jpg, Hajja University, Yemen, edress2020@gmail.com


Rola Jadallahorcid22.jpg, Arab American University, Palestine, rola@aauj.edu


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Keywords: antibiotic resistance, biofilm formation, disc diffusion method, Porphyromonas gingivalis

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How to Cite
Al-Deen, H. S., A. A. M. Al-Ankoshy, M. M. A. Al-Najhi, T. A. Al-Kabsia, K. A. AL-Haddad, A. A. Y. Al-Akwa, H. A. Al-Shamahy, and M. A. Al-labani. “PORPHYROMONAS GINGIVALIS: BIOFILM FORMATION, ANTIMICROBIAL SUSCEPTIBILITY OF ISOLATES FROM CASES OF LOCALIZED AGGRESSIVE PERIODONTITIS (LAP)”. Universal Journal of Pharmaceutical Research, Vol. 6, no. 4, Sept. 2021, doi:https://doi.org/10.22270/ujpr.v6i4.633.
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