BIOFILM FORMATION AND ANTIFUNGAL SUSCEPTIBILITY OF CANDIDA ISOLATES FROM ORAL CAVITY AFTER THE INTRODUCTION OF FIXED ORTHODONTIC APPLIANCES

  • Abdalhaq Hussin Alhasani Orthodontics, Pedodontics and Prevention Department Faculty of Dentistry, Ibb University, Yemen.
  • Ameen Abdullah Yahya Al-Akwa Orthodontics, Pedodontics and Prevention Department Faculty of Dentistry, Sana'a University, Yemen.
  • Hassan Abdul wahab Al-Shamahy
  • Hussein Mohammad Shoga Al-deen Orthodontics, Pedodontics and Prevention Department Faculty of Dentistry, Sana'a University, Yemen.
  • Mohammed A Al-labani Orthodontics, Pedodontics and Prevention Department Faculty of Dentistry, Sana'a University, Yemen.

Abstract

Background and aims: Fixed Orthodontic Appliances (FOA) act as diverse impact zones and modify microbial adherence and colonization, acting as unfamiliar reserves and potential sources of infection  This study was conducted to inspect the effect of the introduction of fixed orthodontic appliances on the growth and adherence (biofilm formation) of Candida species. And also to reveal the species distribution and antifungal sensitivity to isolated Candida species. 


Material and methods: The trial group was chosen   from orthodontic patients whom were inspected clinically as soon as to get baseline information prior to active treatment. The group included 210 patients; 165 females, and 45 males, (mean age 21.6 ± 4.5 years). Clinical, demographic, and risk factor data were collected in a standard questionnaire and then each individual was directed to perform an oral rinse with phosphate-buffered saline, which was expectorated and processed proposed for the isolation and identification of Candida species by standard methods. After that, the isolated Candida species were tested for biofilm production by the phenotypic method i.e. Tissue culture palate methods (TCPM). Finally, antibiogram susceptibility pattern of oral Candida species was done by Kirby-Bauer disc diffusion method for amphotericin B, ketoconazole, and fluconazole.


Results: The most common yeast colonized oral cavity after the introduction of FOA was C. albicans (72.5%), followed by C. glabrata and C. tropicalis  (12.5%), while Candida parapsilosis  only was 2.5%. The rate of formation of biofilms was 52.5% for all types of Candida, and it was found that biofilm formation occurs more frequently among C. tropicals  and C. glabrata (60%) than C. albicans (48.3%). All Candida species isolates were sensitive to amphotericin B and ketoconazole while resistance to fluconazole was found in 40% of C. tropicalis and 20% in C. glabrata  and 13.8% in C. albicans


Conclusion: The present study proved that C. albicans is still the major isolate from the oral cavity after the introduction of FOA, but non-albicans species colonization is raised and FOA might be a factor for biofilm formation. The C. tropicalis and C. glabrata were more– biofilm-producers compared to C. albicans. The isolated species in the current study are less susceptible to fluconazole and drug resistant factor in the Candida species isolates was found to be associated with  biofilm formation.


                      r51.gif


Google_scholar_image3.JPG


Peer Review History:


Received 24 July 2020; Revised 13 August; Accepted 4 September, Available online 15 September 2020


UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.


Received file:blue_23983.gif                Reviewer's Comments:download_logo_r_29189.gif


Average Peer review marks at initial stage: 6.5/10


Average Peer review marks at publication stage: 8.0/10


Reviewer(s) detail:


Dr. Wadhah Hassan Edreesorcid2.jpg, Hajjah University, Yemen, edress2020@gmail.com


Dr. Muhammad Zahid Iqbalorcid2.jpg, AIMST University, Kedah Darul Aman, Malaysia, drmmziqbal@gmail.com


Similar Articles:


ORAL CANDIDA ALBICANS COLONIZATION RATE IN FIXED ORTHODONTICS PATIENTS


ORAL C. ALBICANS COLONIZATION AND NON-CANDIDA ALBICANS CANDIDA COLONIZATION AMONG UNIVERSITY STUDENTS, YEMEN


PREVALENCE AND ASSOCIATED FACTORS OF ORAL NON-CANDIDA ALBICANS CANDIDA CARRIAGE IN DENTURE WEARERS IN SANA'A CITY- YEMEN

Keywords: Antifungal resistance, biofilm formation, Candida species, Candida albicans, Fixed Orthodontic Appliances (FOA), non-albicans species, oral cavity

Downloads

Download data is not yet available.
Comments

Statistics
93 Views | 119 Downloads
How to Cite
Alhasani, A. H., A. A. Y. Al-Akwa, H. A. wahab Al-Shamahy, H. M. S. Al-deen, and M. A. Al-labani. “BIOFILM FORMATION AND ANTIFUNGAL SUSCEPTIBILITY OF CANDIDA ISOLATES FROM ORAL CAVITY AFTER THE INTRODUCTION OF FIXED ORTHODONTIC APPLIANCES”. Universal Journal of Pharmaceutical Research, Vol. 5, no. 4, Sept. 2020, doi:https://doi.org/10.22270/ujpr.v5i4.435.
Section
Research Articles

Most read articles by the same author(s)

1 2 > >>