TOXICITY AND ANTITRYPANOSOMAN ACTIVITY OF HEMI SYNTHESIS PRODUCTS OBTAINED FROM BIOACTIVE COMPOUNDS OF MITRACARPUS SCABER HARVESTED SOUTH OF BENIN

  • BAMBOLA Bouraïma Pharmacognosie Laboratory / Institute of Research and Experimentation in Traditional Medicine and Pharmacopoeia (IREMPT) / Benin Center for Scientific Research and Innovation (CBRSI). 01 BP 06 Oganla Porto-Novo
  • HOUNGBEME Gouton Alba Pharmacognosie Laboratory / Institute of Research and Experimentation in Traditional Medicine and Pharmacopoeia (IREMPT) / Benin Center for Scientific Research and Innovation (CBRSI). 01 BP 06 Oganla Porto-Novo
  • GANDONOU Dossa Clément Laboratory of Enzymology and Biochemistry of Proteins, Faculty of Science and Technology (FAST), University of Abomey-Calavi, 01BP: 188, Cotonou, Benin.
  • GBAGUIDI Ahokannou Fernand Pharmacognosie Laboratory / Institute of Research and Experimentation in Traditional Medicine and Pharmacopoeia (IREMPT) / Benin Center for Scientific Research and Innovation (CBRSI). 01 BP 06 Oganla Porto-Novo. https://orcid.org/0000-0002-4899-0408
  • FAGBOHOUN Louis Pharmacognosie Laboratory / Institute of Research and Experimentation in Traditional Medicine and Pharmacopoeia (IREMPT) / Benin Center for Scientific Research and Innovation (CBRSI). 01 BP 06 Oganla Porto-Novo. https://orcid.org/0000-0002-7063-8304
  • GLINMA Bienvenu Laboratory of Physical and Synthetic Organic Chemistry (LaCOPS), Faculty of Science and Technology (FAST) University of Abomey-Calavi (UAC), BP: 526 Cotonou, Benin. https://orcid.org/0000-0002-0487-4704

Abstract

Present work involve study of the toxicity and the antitrypanosomal activity of the hemi syntheses (total of thiosemicarbazones) extracted from the substrates (reagents of thiosemicarbazides) in 1N hydrochloric acid medium from Mitracarpus Scaber harvested in the region of Abomey-calavi. The ethanolic, dichloromethane and hydroethanol extracts (50:50 v/v) yielded the alkaloid extracts with yields of 4.44%, 2.48% and 5.08% respectively leading to three products of hemi-synthesis P1, P2 and P3 whose larval toxicities have respective values LC50: 78.1μg/mL, 95μg/mL, and 48.8 μg/mL whereas the toxicity tests of the alkaloid samples of ethanol, dichloromethane and hydroethanolic E1, E2 and E3 extracted from the evaluation of the same larval toxicity test gave values of 83.41μg/mL 102.51μg/mL and 52.91μg/mL respectively. These results were less toxic than those of semisynthetic products. Acute and sub-acute toxicity in non-pregnant NMRI female mice after oral gavage of P2 product has been shown to be non-toxic. The antitrypanosomal test was carried out according to the Alamar blue method, it revealed that P1 moderately inhibits trypanosome parasites (IC50=18.06 μg/mL) as well as P2 and P3 with a respective IC50 of 17.24 μg/mL and 20, 68 μg/mL while the alkaloid totals had lower antitrypanosomal activity than the hemi-synthesis products.


Peer Review History:


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Received file


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Average Peer review marks at initial stage: 6.0/10


Average Peer review marks at publication stage: 8.5/10


Reviewer(s) detail:


Name: Dr. Gehan Fawzy Abdel Raoof Kandeel


Affiliation: Pharmacognosy Department, National Research Centre, Dokki, 12622,  Giza, Egypt


E-mail: gehankandeel9@yahoo.com


 


Name: Dr. Dalia Kamal Zaffar Ali 


Affiliation: Modern University for technology and information, Egypt


E-mail: dr.moda88@gmail.com


Comments of reviewer(s):


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Keywords: Antitrypanosomal, hemi synthesis, Mitracarpus scaber, thiosemicarbazide, toxicities
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How to Cite
Bouraïma, B., H. G. Alba, G. D. Clément, G. A. Fernand, F. Louis, and G. Bienvenu. “TOXICITY AND ANTITRYPANOSOMAN ACTIVITY OF HEMI SYNTHESIS PRODUCTS OBTAINED FROM BIOACTIVE COMPOUNDS OF MITRACARPUS SCABER HARVESTED SOUTH OF BENIN”. Universal Journal of Pharmaceutical Research, Vol. 4, no. 5, Nov. 2019, doi:https://doi.org/10.22270/ujpr.v4i5.314.
Section
Research Articles