THE ASSOCIATION BETWEEN LEVELS OF HEPCIDIN, IRON STATUS AND MICRO-INFLAMMATION MARKERS AMONG HAEMODIALYSIS

  • Ebtesam Ahmad Mufadhal Departement of Biochemistry, Faculty of Medicine and Health Sciences, Sana’a University, Republic of Yemen. https://orcid.org/0000-0001-9556-4252
  • Fairouz Kaid Al-Showafi Departement of Biochemistry, Faculty of Medicine and Health Sciences, Sana’a University, Republic of Yemen.
  • Hassan A. Al-Shamahy Medical Microbiology and Clinical Immunology, Faculty of Medicine and Health Sciences, Sana’a University, Republic of Yemen. https://orcid.org/0000-0001-6958-7012
  • Ebtesam Mhdi Al-zabidi Departement of Biochemistry, Faculty of Medicine and Health Sciences, Sana’a University, Republic of Yemen.

Abstract

Hepcidin is a polypeptide that regulates iron homeostasis and could serve as an indicator of functional iron deficiency in patients with end-stage renal disease (ESRD); this may also aid in the assessment of patient's response to erythropoietin (EPO). Erythropoietin is a cytokine glycoprotein secreted by the kidney in response to cellular hypoxia; it stimulates the production of red blood cells (erythrocytes) in the bone marrow. The present study was aimed to investigate serum levels of hepcidin, iron status and inflammation markers such as C-reactive protein (CRP) in patients with ESRD on maintenance HD and to observe the correlation of serum hepcidin with conventional iron and inflammatory markers. A total of 59 patients on maintenance HD were enrolled; 29 age and sex-matched healthy subjects were included as controls. Laboratory tests including complete blood count, creatinine, urea, albumin, BUN, serum hepcidin, serum ferritin, serum iron and CRP were performed. The serum hepcidin levels was measured by a competitive enzyme-linked immunosorbent assay (C-ELISA). Serum hepcidin levels were significantly higher in patients with ESRD than in the control group (63.7±47.4 ng/mL: 11.5± 26.3 ng/mL respectively P<0.001). The hemoglobin and serum iron levels in the patient group were significantly lower than in the control group. Higher feritine levels were found in hemodialysis patients (448.5±710 ng/mL): ( 98.3±83 ng/mL) of controls (P =0.01). A positive and significant correlation was observed between the values of serum hepcidin and CRP.  Serum hepcidin and high-sensitivity C-reactive protein levels were significantly higher in maintenance haemodialysis patients (case=21.2±28.6 mg/L:control=2.9±2.7 mg/L, P=0.001). In conclusion, higher hepcidin levels are found in ESRD patients and serum hepcidin levels are associated with iron status and micro-inflammation (defined as hsCRP < 6mg/l, in maintenance haemodialysis patients). Also, our findings suggest that hepcidin might play a role in the pathophysiology of anemia associated with chronic diseases as ESRD. As well as, ELISA method for measuring serum hepcidin should facilitate the routine measurement of hepcidin in clinical practice.


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Received file


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Average Peer review marks at initial stage: 5.5/10


Average Peer review marks at publication st0ge: 8.5/10


Reviewer(s) detail:


Name: Hebatallah Ahmed Mohamed Moustafa


Affiliation: Heliopolis University Cairo, Egypt


E-mail: anasweety2003@yahoo.com


 


Name: Dr. Heba M. Abd El-Azim 


Affiliation: Damanhour University, Egypt


E-mail: h_m_abdelazim@hotmail.com


Comments of reviewer(s):


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Keywords: ESRD, Ferritin, Hepcidin, Hemodiylasis, renal failure, Yemen
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How to Cite
Mufadhal, E. A., F. K. Al-Showafi, H. A. Al-Shamahy, and E. M. Al-zabidi. “THE ASSOCIATION BETWEEN LEVELS OF HEPCIDIN, IRON STATUS AND MICRO-INFLAMMATION MARKERS AMONG HAEMODIALYSIS”. Universal Journal of Pharmaceutical Research, Vol. 4, no. 5, Nov. 2019, doi:https://doi.org/10.22270/ujpr.v4i5.313.
Section
Research Articles