ANTI-DIARRHEAL ACTIVITY OF ETHANOL AND CHLOROFORM SEED EXTRACT OF COLA NITIDA IN EXPERIMENTALLY INDUCED DIARRHEA

  • Peace Doe Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.
  • Vivien Chinenye Mshelia Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana. https://orcid.org/0000-0002-4603-2660
  • Duke Bright Otchere Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana. https://orcid.org/0000-0001-7860-9308
  • Omar Fofana Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.
  • Audrey Antwiwaa Amissah Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.
  • Persis Nadine Yeboah Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.
  • Harriet Naa Lamptey Mills Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.
  • Ebenezer Ansah-Abrokwah Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana. https://orcid.org/0000-0001-7907-6750
  • Noah Kofi Ametepey Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana. https://orcid.org/0000-0002-4427-616X

Abstract

Cola nitida has been used in traditional medicine to treat diverse ailments including diarrhea. This study is carried out to investigate the antidiarrheal activity of the ethanol and chloroform seed extract of Cola nitida in Wistar albino rats. The ethanol and chloroform extracts of Cola nitida were evaluated with different doses (100mg/kg, 300mg/kg and 650mg/kg of animal weight) orally for antidiarrheal activity using castor oil induced-diarrhea, gastrointestinal motility test and castor oil-induced gastroenteropooling in Wistar albino rats. The observed activity was compared to standard antidiarrheal drug Lopermaide hydrochloride (2mg/kg) and to distilled water (10ml/kg) which served as the negative control. Cola nitida ethanol extract at 150, 300 and 650mg/kg showed 55.64%, 59.73%, and 71.34% inhibition in gastrointestinal motility respectively. A significant reduction in diarrheal episodes (p<0.0001) was also observed with 650mg/kg of both extracts showing 100% inhibition. A reduction in the volume of fluid in the small intestine was also seen, this was however not significant. The chloroform extract of Cola nitida on the other hand produced a significant reduction in volume and weight of small intestinal content (p<0.05) with 650mg/kg showing a 92.73% inhibition of intestinal fluid accumulation. The ethanol and chloroform extract of Cola nitida showed anti-diarrheal activity in animal model by decreasing the frequency of defecation and by reducing gastrointestinal motility and intraluminal fluid accumulation in the intestine.


Peer Review History:


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Received file


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Average Peer review marks at initial stage: 6.0/10


Average Peer review marks at publication stage: 8.0/10


Reviewer(s) detail:


Name: Prof. Dr. Hüsniye Kayalar


Affiliation: Ege University, Turkey


E-mail: husniyekayalar@gmail.com


 


Name: Dr. Ali Gamal Ahmed Al-kaf


Affiliation: Sana'a university, Yemen


E-mail: alialkaf21@gmail.com


Comments of reviewer(s):


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Keywords: Anti-diarrheal, Cola nitida, gastro-intestinal motility, seeds
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How to Cite
Doe, P., V. C. Mshelia, D. B. Otchere, O. Fofana, A. A. Amissah, P. N. Yeboah, H. N. L. Mills, E. Ansah-Abrokwah, and N. K. Ametepey. “ANTI-DIARRHEAL ACTIVITY OF ETHANOL AND CHLOROFORM SEED EXTRACT OF COLA NITIDA IN EXPERIMENTALLY INDUCED DIARRHEA”. Universal Journal of Pharmaceutical Research, Vol. 4, no. 5, Nov. 2019, doi:https://doi.org/10.22270/ujpr.v4i5.312.
Section
Research Articles