• Gehan F. Abdel Raoof Pharmacognosy Department, National Research Centre, Dokki, 12622, Giza, Egypt
  • Hala M. Mohammed Home Economics department (Nutrition & Food Science), Faculty of Agricultue, Cairo University, Egypt.


The aim of this research was to investigate the chemical composition of petroleum ether extract of Tilia cordata aerial parts as well as to evaluate its cytotoxic activity. Gas chromatography and gas chromatography–mass spectrometry (GC-MS) were used to analyze the unsaponifiable matter and fatty acid methyl esters. Moreover, the cytotoxicity was examined against human hepatoma HepG2 cell line and breast adenocarcinoma MCF cell line. The result showed that thirteen compounds were identified in the fatty acid methyl esters fraction representing 93.71% of the total identified peak area. The major compounds were Octadecanoic acid methyl ester (36.26%) and eicosanoic acid methyl ester (29.42%), whereas nineteen compounds in the unsaponifiable fraction were identified representing 90.56 % of the total beak area. The major compounds were 1- Nonene (30.44%), 1-Hexadecene (24.83%) and phytol (10.40%). Moreover, petroleum ether extract showed a potent cytotoxic effect against human hepatoma HepG2 cell line and a moderate cytotoxic effect on breast adenocarcinoma MCF7 human tumor cell line. So the current research aims to be the first step toward the use of petroleum ether extract of Tilia cordata aerial parts as a potent cytotoxic drug.

Peer Review History:

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Received file


Average Peer review marks at initial stage: 8/10

Average Peer review marks at publication stage: 9.5/10

Reviewer(s) detail:

Name: Dr. Mohamed Said Fathy Al-Refaey

Affiliation: University of Sadat City, Menofia, Egypt



Name: Dr. Ali Gamal Ahmed Al-kaf

Affiliation: Sana'a University, Yemen



Name: Prof. Dr. Hüsniye Kayalar

Affiliation: Ege University, Turkey


Comments of reviewer(s):


Keywords: Aerial parts, chemical composition, cytotoxicity, petroleum ether extract, Tilia cordata
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How to Cite
Raoof, G. F. A., and H. M. Mohammed. “CYTOTOXIC EFFECT AND PHYTOCHEMICAL STUDY OF PETROLEUM ETHER EXTRACT OF TILIA CORDATA MILL”. Universal Journal of Pharmaceutical Research, Vol. 4, no. 4, Sept. 2019, doi:
Research Articles